University of Southampton

We have over 20 years experience in the use of immunohistochemical techniques. The HRU has a range of validated protocols suitable for frozen, paraffin and GMA resin sections. We stock a broad range of primary antibodies including those to identify cells, adhesion molecules, epithelial components, assess cell proliferation status and cytokines optimised for the study of inflammatory processes in a variety of tissues. We also have the secondary antibodies and detection kits for these protocols.

We have also optimised double staining using either two primary antibodies or an antibody in combination with a tinctorial stain.

Paraffin section of tonsil stained for CD20.

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GMA section of nasal polypstained for CD4.

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Nasal polyp double stained for beta tublin (pink) and CDE-1 (brown) [Yoshisue H et al].