Biomedical Imaging Unit

Equipment

Hitachi H7000 Transmission Electron Microscope

  • 125 Kv / 600,000x magnification
  • plate camera
  • digital imaging to 1376x1032 pixels
  • goniometer stage

H7000 

FEI Technai12 Transmission Electron Microscope

  • 120 Kv / 500,000 magnification
  • tilting specimen holder
  • EDAX X-ray microanalysis
  • digital imaging to 1376x1032 pixels, with 5x5 frame auto-montage facility
  • goniometer stage

T12 

FEI Quanta 200 Scanning Electron Microscope

  • 30 Kv / 100,000x magnification
  • high vacuum detector
  • low vacuum detector
  • environmental detector
  • backscatter detector
  • EDAX X-ray microanalysis
  • digital imaging to 3584x3094 pixels

Quanta200 

Electron Microscopy Sample Processing

  • processing laboratories with fume cupboard, balance, sample rotators, embedding ovens etc.
  • 2 x Reichurt Om-U3 ultramicrotomes
  • 2 x Reichurt Ultracut E ultramicrotomes
  • 2 x LKB knife makers
  • Balzers CPD 030 critical point drier
  • Polaron E5100 sputter coater
  • photographic darkrooms with enlargers and auto processors

separate processing and ultramicrotomy facilities for diagnostic samples

ultamicrotome  

Leica SP5 Laser Scanning Confocal Microscope

  • inverted Leica DMI600 stand
  • 9 laser lines (405 to 633 nm)
  • 4 spectral detectors and transmitted light detector to >8000 pixel linear resolution
  • objective range x5 to x100 with optical zoom
  • DIC and phase contrast imaging
  • environmental chamber and 5% CO2 in air supply
  • resonant scanner
  • electronic stage with relocation and tile scan function
  • standard fluorescence microscopy with FITC / TRITC / DAPI compatible filter cubes
  • 4 MP colour digital camera 

SP5 

Leica SP2 Laser Scanning Confocal Microscope

  • upright Leica DM RBE stand
  • 5 laser lines (405 to 633 nm)
  • 4 spectral detectors and transmitted light detector to > 8000 pixel linear resolution
  • objective range x5 to x100 with optical zoom, incl. x50 dipping lens
  • DIC imaging
  • galvo stage for fast XZ imaging
  • standard fluorescence microscopy with FITC / TRITC compatible filter cubes 

SP2 

Olympus IX81 Live Cell Imaging System

  • inverted Olympus IX81 automated stand
  • objective range x4 to x60
  • phase contrast imaging
  • environmental chamber and CO2 controller
  • standard fluorescence microscopy with FITC / TRITC / DAPI compatible filter cubes
  • electronic stage with relocation function
  • combined phase-fluorescence time lapse facility
  • 1376 x 1032 pixel high sensitivity monochrome camera for time lapse and fluorescence photomicroscopy
  • 4 MP colour digital camera 

IX81  

Olympus dotSlide Virtual Microscopy System

  • Olympus BX61 microscope frame
  • automated slide scanning / virtual microscopy system with high quality, colour microscope camera
  • brightfield imaging
  • objective range x4 to x60
  • web site generation for tiled image sets 

dotSlide  

Nikon Eclipse E600 Photomicroscope

  • brightfield and polarising imaging
  • objective range x2 to x100
  • 4 MP colour digital camera

Eclipse E600  

Olympus  SZX9 Low Power Photomicroscope

  • continuous zoom from x6.3 to x 57
  • transmitted and fibre optic ring incident lighting
  • Oblique illumination fluorescence illumination with 9 filter sets
  • 4 MP colour digital camera 

SZX 

Other equipment:

  • Multi-terabyte secure server for data backup and archiving more info
  • Image analysis workstation
  • Epson stylus photo R2400 A3 photo printer
  • Epson Perfection V700 flatbed scanner
  • Lexmark colour laser printer
  • Polaroid Pro Palette 800 35mm slide writer
  • Coming soon! Zeiss photomicroscope with phase contrast and darkfield optics

Notes:

  • Instrument time on our electron microscopes, confocals and timelapse system is charged for in order to cover the costs of service contacts, repairs, upgrades, reagents and consummables. Access charges should be costed into, and itemised on, all future University of Southampton grant applications at full economic cost. For current charges for access and services, and advice on including facility access in grant applications please contact Anton Page email 
  • After tuition, users are expected to drive our instruments for themselves. Microscope tuition is not charged for and help and advice are always available.
  • Sample processing charges only apply if this is carried out by BIU staff on behalf of users. A charge is not usually made for users who process their own material. After tuition, students will be expected to do all of their own processing and cutting and therefore will not be charged for this.
  • The Imaging and Microscopy Centre in the new Institute for Life Sciences on Highfield Campus has additional confocal instruments: a Leica TCS SP2 multiphoton system and a Zeiss LSM510 UV laser system.


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