Equipment :: Biomedical Imaging Unit - Research - Faculty of Medicine - University of Southampton, Faculty of Medicine

Hitachi H7000 Transmission Electron Microscope

FEI Technai12 Transmission Electron Microscope

FEI Quanta 200 Scanning Electron Microscope

Quanta200

Electron Microscopy Sample Processing

processing laboratories with fume cupboard, balance, sample rotators, embedding ovens etc.
2 x Reichurt Om-U3 ultramicrotomes
2 x Reichurt Ultracut E ultramicrotomes
2 x LKB knife makers
Balzers CPD 030 critical point drier
Polaron E5100 sputter coater
photographic darkrooms with enlargers and auto processors

separate processing and ultramicrotomy facilities for diagnostic samples

ultamicrotome

Leica SP5 Laser Scanning Confocal Microscope

inverted Leica DMI600 stand
9 laser lines (405 to 633 nm)
4 spectral detectors and transmitted light detector to >8000 pixel linear resolution
objective range x5 to x100 with optical zoom
DIC and phase contrast imaging
environmental chamber and 5% CO₂ in air supply
resonant scanner
electronic stage with relocation and tile scan function
standard fluorescence microscopy with FITC / TRITC / DAPI compatible filter cubes
4 MP colour digital camera

SP5

Leica SP2 Laser Scanning Confocal Microscope

upright Leica DM RBE stand
5 laser lines (405 to 633 nm)
4 spectral detectors and transmitted light detector to > 8000 pixel linear resolution
objective range x5 to x100 with optical zoom, incl. x50 dipping lens
DIC imaging
galvo stage for fast XZ imaging
standard fluorescence microscopy with FITC / TRITC compatible filter cubes

SP2

Olympus IX81 Live Cell Imaging System

inverted Olympus IX81 automated stand
objective range x4 to x60
phase contrast imaging
environmental chamber and CO₂ controller
standard fluorescence microscopy with FITC / TRITC / DAPI compatible filter cubes
electronic stage with relocation function
combined phase-fluorescence time lapse facility
1376 x 1032 pixel high sensitivity monochrome camera for time lapse and fluorescence photomicroscopy
4 MP colour digital camera

IX81

Olympus dotSlide Virtual Microscopy System

Olympus BX61 microscope frame
automated slide scanning / virtual microscopy system with high quality, colour microscope camera
brightfield imaging
objective range x4 to x60
web site generation for tiled image sets

dotSlide

Nikon Eclipse E600 Photomicroscope

Eclipse E600

Olympus SZX9 Low Power Photomicroscope

SZX

Other equipment:

Instrument time on our electron microscopes, confocals and timelapse system is charged for in order to cover the costs of service contacts, repairs, upgrades, reagents and consummables. Access charges should be costed into, and itemised on, all future University of Southampton grant applications at full economic cost. For current charges for access and services, and advice on including facility access in grant applications please contact Anton Page
After tuition, users are expected to drive our instruments for themselves. Microscope tuition is not charged for and help and advice are always available.
Sample processing charges only apply if this is carried out by BIU staff on behalf of users. A charge is not usually made for users who process their own material. After tuition, students will be expected to do all of their own processing and cutting and therefore will not be charged for this.
The Imaging and Microscopy Centre in the new Institute for Life Sciences on Highfield Campus has additional confocal instruments: a Leica TCS SP2 multiphoton system and a Zeiss LSM510 UV laser system.

University of Southampton